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Culture and identification of spinal motor neurons, as well as determination of GPR30 expression in these cells (×200). (A) Spinal motor neurons thawed after 72 h. A number of projections may be observed and visible links exist between the projections. (B) and (C) Double immunofluorescent staining: Green represents the motor neuron marker, SMI32, while red represents GPR30. GPR30, G-protein-coupled receptor 30.
