Figure 5.

Effect of triptolide ex vivo in the isolated subepithelial myofibroblasts from the colon of rats with chronic colitis and colonic fibrosis. (A) Collagen Iα1 transcripts in isolated colonic subepithelial myofibroblasts were measured by reverse transcription-quantitative polymerase chain reaction. Results are expressed as the mean ± standard error for the samples from 10 rats/group. ^*P<0.05; ^**P<0.01 and ^***P<0.001. (B) Collagen I protein in isolated colonic subepithelial myofibroblasts was measured by immunoblot analysis. Results are from the representative experiment. NS, no significance; PBS, phosphate-buffered saline; Trip, triptolide TNBS, 2,4,6-trinitrobenzene sulfonic acid.