Fig. 3.

Breast tumor downregulation of Cx43 in vSMC is time- and dose- dependent. a, b. vSMC were stimulated for 24 h with media conditioned by the indicated cell line and lysates analyzed for Cx43 levels. Bottom panels represent quantification of an = 4 experiments, bn = 5 experiments, *p < 0.05; **p < 0.01; ***p < 0.001. c. vSMC were treated for the indicated time with media conditioned by MDA-MB-231 cells and lysates analyzed by Western. Data representative of two to four experiments. d vSMC were treated for 24 h with media conditioned by varying dilutions of MDA-MB-231 cell CM. Bottom panel represents quantification from 3 independent experiments. e. mRNA was isolated and subjected to quantitative PCR analysis. Data were normalized to GAPDH levels and fold change compared to Mock; data represent the mean of three independent experiments each performed in technical triplicate (*p < 0.05; **p < 0.01)