Figure 2.
Antigen-presenting ability of PMDC11 and caTLR-PMDC11 cells. Antigen-presenting abilities of these stimulator cells were assayed by allogeneic mixed leukocyte culture using 3H-thymidine incorporation. Graded numbers of PMDC11, LPS-stimulated PMD11, caTLR4-PMDC11 or doxycycline-exposed caTLR4-PMD11 cells were used as stimulator cells and normal peripheral blood mononuclear cells (1×105/well) were the responder cells. Statistical differences between PMDC11 cells and other stimulator cells were evaluated by two-way analysis of variance. The values are expressed as the mean ± standard error of the mean. The two experiments not presented had similar results. cpm, count per minute; LPS, lipopolysaccharide; Dox, doxycycline.