Figure 3.
IFN-γ production of PB-MNCs stimulated with PMDC11 or caTLR-PMDC11 cells. The IFN-γ concentration of MLC supernatant was assayed using the cytometric bead array human IFN-γ Flex Set Assay. A total of 1×104 cells/well of PMDC11, LPS-stimulated PMD11, caTLR4-PMDC11 or doxycycline-exposed caTLR4-PMD11 cells were used as stimulator cells and 1×105 cells/well of normal PB-MNCs were used as responder cells in MLC over 5 days. The bar graph shows the mean concentration of IFN-γ. Statistical differences between groups were evaluated using one-way analysis of variance. The values are expressed as the mean ± standard error of the mean. IFN, interferon; PB-MNCs, peripheral blood mononuclear cells; MLC, mixed leukocyte culture; LPS, lipopolysaccharide; Dox, doxycycline.