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Dual-functionalization of TTL: A) MALDI–MS spectra (red: modified protein (as marked below); black: reference protein AhaSer-TTL[Aha]; m/z (calculated): [M+H]+ 31245 Da; for full spectra see Supporting Information File 1) B) SDS PAGE of TTL protein conjugates (Coomassie stain), C) Western Blot (streptavidin-peroxidase antibody).
