Table I.
Polymerase chain reaction primer and TaqMan® probe sequences of each gene of interest.
| Gene | Primer | Primer sequence | Amplification segment (bp) |
|---|---|---|---|
| IDO | F R TM |
5′-TGGCAAACTGGAAGAAAAAG-3′ 5′-ATTGCTTTGGATTGCAGGAGAA-3′ 5′-TTTCCTGGTGGGGACTGCGA-3′ |
151 |
| HO-1 | F R TM |
5′-GACAGCATGTCCCAGGATTT-3′ 5′-CATCACCAGCTTAAAGCCTT-3′ 5′-CACCTCCTTGGTGGCCTCCTTC-3′ |
135 |
| IL-7 | F R TM |
5′-CATCAATCAACTGGACAAAATG-3′ 5′-GTCATTGAATTCCTCACTGAT-3′ 5′-CCTCAACTTGCGAGCAGCAC-3′ |
172 |
| GAPDH | F R TM |
5′-CCTCAAGATTGTCAGCAAT-3′ 5′-CCATCCACAGTCTTCTGAGT-3′ 5′-FAM-ACCACAGTCCATGCCATCAC-TAMRA-3′ |
141 |
F, forward primer; R, reverse primer; TM, TaqMan probe; IDO, indoleamine 2,3-dioxygenase; HO-1, heme oxygenase-1; IL-7, interleukin-7; bp, base pairs.