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Necrotic zone assay of rat brain tissue sections stained with TTC. (A) Brain sections stained with TTC from the sham, 0 h reperfusion and 32 h reperfusion groups. (B) Histogram for comparison of the necrotic zones in different brain sections, indicating that necrotic zone areas increased with increasing reperfusion time and different brain slices exhibited different necrotic zone (P<0.01). TTC, Triphenyltetrazolium chloride.
