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. 2015 Apr 20;12(2):1753–1760. doi: 10.3892/mmr.2015.3645

Figure 4.

Figure 4

Effects of MSCs on protein and gene expression levels in chondrocytes incubated with IL-1β. The three groups represent chondrocytes without treatment (con) and chondrocytes co-cultured with (IL+MSCs) or without (IL) MSCs for three days (for analysis of Col2 and aggrecan) or for one day (for COX-2 and MMP-13 analysis) following incubation with 10 ng/ml IL-1β for 24 h, respectively. (A) Protein expression was analyzed by western blotting and the bands shown are representative of typical results. Gene expression levels of (B) Col2, (C) aggrecan, (D) MMP-13 and (E) COX-2 were analyzed by RT-qPCR. Histograms represent the mean of values relative to the maximum value. RT-qPCR analyses were run in triplicate and the results are presented as the mean ± standard deviation. *P<0.05 vs. control group; #P<0.05 vs. IL group. MSCs, mesenchymal stem cells; IL-1β, interleukin-1β; Col2, type II collagen; COX-2, cyclooxygenase-2; MMP-13, matrix metalloproteinase-13.