Figure 2.

Induction of autophagy in EC9706 human esophageal squamous carcinoma cells by radiation. Cells were treated with irradiation and autophagic levels were detected. LC3 and beclin-1 protein expression levels were determined by western blot analysis. The ratios of LC3-I, LC3-II and beclin-1 to actin levels were determined densitometrically. The results are the averages of triplicate samples. Error bars represent the standard error. ^*P<0.05 vs. IR alone. 3-MA, 3-methyladenine; IR, ionizing radiation; LC3, microtubule-associated protein light chain 3.