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. 2015 Apr 16;12(2):1645–1652. doi: 10.3892/mmr.2015.3623

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Inhibition of autophagy induces G₂/M phase cell cycle arrest. (A) Effects of 3-MA, IR or their combination on cell cycle distribution of EC9706 human esophageal squamous carcinoma cells were assessed by flow cytometry. (B) Histograms of flow cytometric analysis. Cell populations with differential propidium iodide staining intensity were evaluated using CellQuest™ software. The results are the averages of triplicate samples. Error bars represent the standard error. 3-MA, 3-methyladenine; IR, ionizing radiation.