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. 2015 Jun 12;5:10009. doi: 10.1038/srep10009

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Horizontal and vertical confocal images of polarized MDCK cells transiently expressing the K_V7.1–WT or MUT as indicated and labeled with antibodies against K_V7.1 (left panels) and Phalloidin (middle panels). Merged pictures are shown in right panels. Ap; apical; Ba, basolateral. Representative pictures from three independent experiments are shown. Quantification of ratio between channels in the membrane versus channels trapped in intracellular compartments (most likely ER) for WT and mutants (p < 0.0001, n = 18−22 cells each situation).