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Irradiation-induced apoptosis in NCI-H520 and NCI-H520/R cells. The cells were seeded and incubated for 48 h following treatment with 10 Gy irradiation. Annexin V-fluorescein isothiocyanate and PI staining was performed, followed by fluorescence-activated cell sorting analysis. The percentage of early apoptotic cells (annexin V+, PI−) was calculated. Similar results were obtained in three independent experiments. PI, propidium iodide.
