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. 2015 Jun 12;5:10465. doi: 10.1038/srep10465

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Expression levels of diverse bacterial LMCOs in E. coli. SDS PAGE of biomass-normalised (OD₆₀₀) total cell protein (TCP) and cell free extract (CFE) samples prepared after overnight induction under static conditions. Samples were denatured for 20 min at 95 °C before loading and proteins were visualised by Coomassie Brilliant Blue staining. Expected molecular masses of recombinant LMCOs: B. coagulans 59.7 kDa, B. clausii 58.4 kDa, S. pristinaespiralis 32.6 kDa, B. subtilis 58.5 kDa, G. forsetii 59.0 kDa, M. tractuosa 59.6 kDa, S. linguale 51.1 kDa.