Table 2. Final optical densities and volumetric activity yields in shake flask cultures (SF) of E. coli expressing bacterial LMCOs.
| Enzyme | Plasmid |
Final optical density (600 nm)±S.D. |
Volumetric activity [μmol min−1
L−1]±S.D. |
||||||
|---|---|---|---|---|---|---|---|---|---|
|
LB |
LBPG | +pRARE2 |
LB |
LBPG | +pRARE2 | ||||
| SF shaken | SF static | SF static | best cond. | SF shaken | SF static | SF static | best cond. | ||
| B. pumilus CotA | pBuL | 3.8 (±0.2) | 1.2 (±0.1) | 1.0 (±0.11) | 1.7 (±0.6) | 12 (±1) | 97 (±19) | 617 (±81) | 4157 (±452) |
| B. subtilis CotA | pLOM10 | 4.5 (±0.2) | 1.5 (±0.1) | 2.4 (±1.0) | 1.2 (±0.1) | 18 (±1) | 526 (±47) | 2648 (±273) | 2612 (±217) |
| B. clausii CotA | pBaL | 2.3 (±0.4) | 1.5 (±0.1) | 1.2 (±0.1) | 1.5 (±0.06) | 8.6 (±1.4) | 60 (±9) | 19 (±2) | 110 (±8) |
| B. coagulans CotA | pBoL | 2.4 (±0.4) | 1.3 (±0.4) | 2.0 (±0.1) | 1.3 (±0.04) | 7.8 (±0.7) | 924 (±133) | 52 (±5) | 719 (±25) |
| G. forsetii LMCO with SP | pGoL1 | n.a. | 2.7 (±0.4) | 2.0 (±0.4) | n.a. | n.a. | 36 (±8) | 26 (±5) | n.a. |
| G. forsetii LMCO w/o SP | pGoL3 | 5.4 (±0.2) | 2.5 (±0.2) | 1.3 (±0.2) | 1.9 (±0.1) | 137 (±12) | 2440 (±244) | 220 (±32) | 422 (±3) |
| M. tractuosa LMCO w/o SP | pMtraL | 4.3 (±0.1) | 1.7 (±0.1) | 1.1 (±0.3) | 1.3 (±0.04) | 30 (±5) | 1615 (±118) | 1356 (±128) | 353 (±47) |
| S. pristinaesp. LMCO with SP | pSpL3 | 6.6 (±0.5) | 1.7 (±0.1) | 2.3 (±0.2) | 1.2 (±0.1) | 57 (±14) | 83 (±7) | 2.7 (±0.7) | 85.5 (±5.7) |
| S. linguale LMCO w/o SP | pSiL | 5.2 (±0.2) | 1.7 (±0.1) | 1.9 (±0.2) | 1.4 (±0.2) | 3.0 (±0.1) | 2.1 (±0.6) | 2.4 (±1.6) | 5.1 (±0.9) |
| w/o=without, SP=signal peptide, S.D.=standard deviation, n.a.=not analysed | |||||||||