. 2015 Jun 12;5:10465. doi: 10.1038/srep10465

Table 3. Substrate range of B. clausii and B. coagulans LMCOs compared to previously characterised B. subtilis CotA (Reiss et al. ⁴). Out of 91 tested compounds only those are shown which were oxidised by at least one of the three enzymes.

Structural group	Compound	B. subt.⁴	B. clau.	B. coag.
Aromatic carboxylic acids	p-Coumaric acid	+/−	+	+
	Caffeic acid	+	−	−
	Ferulic acid	+	+/−	+/−
	Sinapic acid	+	+	+
	3,4-Dihydroxybenzoic acid	+	+/−	+/−
	Gallic acid	+	−	−
	Syringic acid	+	+	+
	3-Amino-4 hydroxybenzoic acid	+	−	+/−
	4-Amino-3 hydroxybenzoic acid	+	+	+
	Vanillic acid	+/−	+/−	+/−
	3-(Dimethylamino) benzoic acid	−	+/−	+
	3-Hydroxyanthranilic acid	+	−	−
	p-Hydroxyphenylpyruvic acid	+	−	−
Aromatic alcohols	Vanillyl alcohol	+	+	+
	Isovanillyl alcohol	+	+	+/−
	Coniferyl alcohol	+	+	+
	Tyrosol	+	−	−
	p-Cresol	+	+/−	−
	2,6-Dimethylphenol	+	−	+
	Catechol	+	+	+
	4-Methylcatechol	+	+	+
	Pyrogallol	+	+/−	+/−
	2,6-Dimethoxyphenol	+	+	+
	3,4,5-Trimethoxyphenol	+/−	−	−
	Guaiacol	+	+	+
	Hydroquinone	+	+	+
	Mesitol	+	+	+/−
	3-Methylcatechol	+	+	+
	Eugenol	+	+	+
	Arbutin	+	+	+
	Resveratrol	+	+/−	+/−
	Quercetin hydrate	+/−	+	+
Aromatic ketones	Acetovanillone	+	+/−	+
	Acetosyringone	+	+/−	+/−
Aromatic aldehydes	o-Vanillin	+	+	+
	Syringaldehyde	+	+/−	+/−
	Ethyl vanillin	+	+	+
	Vanillin	+/−	−	+/−
	Sinapaldehyde	+	+	+
	Coniferyl aldehyde	+	+	+
Aromatic amines	Dopamine hydrochloride	+	+	+
Aromatic esters	Methyl vanillate	+	+/−	+/−
	Methylsyringate	+	−	−
Aromatic amides	Syringamide	+	+	+
	N-Hydroxyacetanilide	+	+	+
Polyphenol	Tannic acid	+	+	+
N-heterocycles	Violuric acid hydrate	−	+/−	+/−
	1-(3-Sulfophenyl)-3-methyl-2-pyrazolin-5-one	+	+	+
	1-(4-Sulfophenyl)-3-methyl-5-pyrazolone	+	+	+
Aromatic azo compounds	ABTS	+	+	+
	Syringaldazine	+	+	+
Triphenyl compounds	Cresol red sodium salt	+	+	+
Chromans	(+)-Catechin hydrate	+	+	+
	(−)-Epicatechin	+	+	+
Phenothiazines	Phenothiazine	+	+	+
	Promazine hydrochloride	+	+	+
Benzonitriles	2,3-Dimethoxybenzonitrile	+/−	−	−
Naphtalenes	1-Nitroso-2-naphthol-3,6-disulfonic acid	+	+	+
	2-Nitroso-1-naphthol-4-sulfonic acid	+	+	+
	1-Amino-2-naphthol-4-sulfonic acid	+	−	+/−

Table 3. Substrate range of B. clausii and B. coagulans LMCOs compared to previously characterised B. subtilis CotA (Reiss et al. 4). Out of 91 tested compounds only those are shown which were oxidised by at least one of the three enzymes.

Table 3. Substrate range of B. clausii and B. coagulans LMCOs compared to previously characterised B. subtilis CotA (Reiss et al. ⁴). Out of 91 tested compounds only those are shown which were oxidised by at least one of the three enzymes.