Figure 3. Effects of 80 % EtOH extract of H. radicata flower (HRF) and T. platycarpum flower (TRF) on protein levels of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells. Cells (1.0 × 106 cells/mL) were pre-incubated for 18 h, and then stimulated with LPS (1 µg/mL) for 24 h in the presence of each of the 80 % EtOH extracts (100, 200, or 400 µg/mL), 2-amino (10 µM 2-amino-4-methylpyridine), or dex (20 µM dexamethasone). The protein levels were determined using Western blotting. 80 % EtOH extract of (A) HRF and (B) TPF.
