Figure 5. Effects of ethyl acetate extract of H. radicata flower (HRF-EA) on the protein levels of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells. Cells (1.0 × 106 cells/mL) were pre-incubated for 18 h, and then stimulated with LPS (1 µg/mL) for 24 h in the presence of HRF-EA (12.5, 25, or 50 µg/mL), 2-amino (10 µM 2-amino-4-methylpyridine), or dex (20 µM dexamethasone). The protein levels were determined using Western blotting.
