Figure 7. Effects of ethyl acetate extract of H. radicata flower (HRF-EA) on p38, JNK, and ERK protein levels in LPS-stimulated RAW 264.7 cells. Cells (1.5 × 106 cells/mL) were pre-incubated for 18 h, incubated with HRF-EA (12.5, 25, and 50 µg/mL) and each of the indi-cated inhibitors (SB [10 µM SB203580], SP [20 µM SP600125], and PD [20 µM PD98059]) for 2 h, and then stimulated with LPS (1 µg/ml) for 30 min. The protein levels were determined using Western blot.
