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. 2015 Apr 16;12(2):1717–1726. doi: 10.3892/mmr.2015.3637

Figure 5.

Figure 5

Combined treatment significantly enhances the OPG/RANKL ratio in the proximal tibia of OVX rats. Effects of ASA, sCT and combined treatment (sCT+ASA) on protein expression levels in the proximal tibia of OVX rats. Specimens were observed at magnification, x250. Antigens were detected using an avidin-biotin-peroxidase system and visualized with 3,3′-diaminobenzidine. Protein levels were measured by densitometry. Values are expressed as the mean ± standard deviation. P-values were calculated by analysis of variance, with Student-Newman-Keuls analysis). (A) OPG; (B) RANKL; (C) OPG/RANKL protein ratio. Positive staining for OPG and RANKL was mainly localized in the chondrocytes of the epiphyseal growth plate and trabeculae of bone. ASA, aspirin; sCT, salmon calcitonin; OVX, ovariectomized; OPG, osteoprotegerin; RANKL, receptor activator of nuclear factor κB ligand.