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. 2015 Apr 1;12(2):1727–1732. doi: 10.3892/mmr.2015.3588

Figure 2.

Figure 2

3-MA efficiently inhibits cisplatin-induced autophagy in U251 cells. (A) U251 cells were treated with cisplatin (10 µg/ml) for 6, 12 and 24 h. The expression of LC3 was detected by confocal microscopy (scale bar, 10 µm; FITC-conjugated secondary antibody). (B) The cells were treated with cisplatin (10 µg/ml) for 6, 12 and 24 h, and the expression of LC3 was detected by western blot analysis. (C) The protein levels were quantified for LC3-II/I. The data are expressed as the mean ± standard deviation (n=3; **P<0.01, vs. control). (D) The cells were treated with cisplatin (10 µg/ml) and/or 3-MA (10 mM) for 12 h, and the expression of LC3 was detected by confocal microscopy (Bar, 10 µm; FITC-conjugated secondary antibody). (E) The cells were treated with cisplatin (10 µg/ml) and/or 3-MA (10 mM) for 12 h, and the expression of LC3 was detected by western blot analysis. (F) Quantitation of the protein expression of LC3-II/I. The data are expressed as the mean ± standard deviation (n=3; **P<0.01, vs. control; #P<0.05, vs. cisplatin). 3-MA, 3-methyladenine; LC3, microtubule-associated protein 1 light chain 3; FITC, fluorescein isothiocyanate.