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. 2015 Apr 1;12(2):1727–1732. doi: 10.3892/mmr.2015.3588

Figure 4.

Figure 4

3-MA increases cisplatin-induced apoptosis by increasing endoplasmic reticulum stress. (A) U251 cells were treated with cisplatin (10 µg/ml) and/or 3-MA (10 mM) for 12 h, and cell viability was determined by MTT assay (**P<0.01, vs. control; #P<0.05, vs. Cisplatin). (B) The cells were treated with cisplatin (10 µg/ml) and/or 3-MA (10 mM) for 12 h, stained with Hoechst 33342 and cell morphology was observed by confocal microscopy (Scale bar, 20 µm). (C) The cells were treated with cisplatin (10 µg/ml) and/or 3-MA (10 mM) for 12 h, and the expression of cleaved caspase-3 was detected by confocal microscopy (Scale bar, 10 µm; Texas red-conjugated secondary antibody). (D) The cells were treated with cisplatin (10 µg/ml) and/or 3-MA (10 mM) for 12 h, and western blot analysis was performed to determine the expression levels of CHOP, caspase-4, cleaved caspase-4, caspase-3 and cleaved caspase-3. (E) Quantitation of the proteins level. The data are expressed as the mean ± standard deviation (n=3; **P<0.01, vs. control; #P<0.05, vs. cisplatin). 3-MA, 3-methyladenine; CHOP, CCAAT-enhancer-binding protein homologous protein.