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. 2015 May 7;12(2):2622–2628. doi: 10.3892/mmr.2015.3740

Figure 4.

Figure 4

HDM2 interacts with p53 in CCRCC. (A) Protein levels of p-AKT (Ser473), T-AKT, p-HDM2, HDM2 and p53 in non-tumor kidney tissues (normal, N1–N5) and CCRCC (T1–T5). GAPDH was used as the internal control. (B) Co-immunoprecipitation of HDM2 in normal cells or CRCC were precipitated for HDM2 and immunoblotted for p53, p-HDM2 and HDM2. (C) Representative micrographs of immunohistochemical staining for p-AKT (Ser473), p-HDM2 and p53 in non-tumor kidney tissues and CCRCC (magnification, x100). CCRCC, clear-cell renal cell carcinoma; p, phosphorylated; T, total; IgG, immunoglobulin G; I P, immunoprecipitation.