Figure 2.

Methylation analysis of ADAMTS1 in the six gastric cell lines. (A) Methylation status of ADAMTS1 in the GES-1 normal gastric cell line and five gastric cancer cell lines. (B) Relative mRNA levels of ADAMTS1 in the unmethylated gastric cell lines and methylated gastric cell lines (Mann-Whitney U test; P=0.036). (C) Bisulphite sequence electropherogram of methylated MGC-803. CG, which remained unchanged are underlined in black. Data are presented as the mean ± standard deviation. U, unmethylated; M, methylated; IVD, positive control; H₂O, negative control; ADAMTS1, A disintegrin and metallopeptidase with thrombospondin motif type 1.