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. 2015 Apr 23;12(2):2487–2494. doi: 10.3892/mmr.2015.3667

Figure 4.

Figure 4

mRNA and protein expression levels of ADAMTS1 in the MGC-803 cell line prior to and following treatment with different concentrations of 5-Aza-dC for 3 days. (A) mRNA expression of ADAMTS1 prior to and following treatment with different concentrations of 5-Aza-dC using reverse transcription-quantitative polymerase chain reaction analysis. (B) ADAMTS1 relative mRNA levels prior to and following treatment with different concentrations of 5-Aza-dC (Mann-Whitney U test; ***P<0.001). (C) Protein levels of ADAMTS1 prior to and following treatment with different concentrations of 5-Aza-dC, detected using immunofuorescence (magnification, x200). The MGC-803 cells immunostained with antibody to ADAMTS1 (green) were characterized by perinuclear cytoplasmic immunofluorescence. The cell nuclei were visualized with Hoechst33258 (blue). (D) Representative figures of MGC-803 cell invasion in untreated and 5-Aza-dC-treated MGC-803 cells. (a) Invasion in the untreated group; (b) invasion in the 5-Aza-dC-treated group. (E) Numbers of MGC-803 cells following treatment with 5-Aza-dC compared with the untreated control group. The values are presented as the mean ± standard deviation (Mann-Whitney U test; P<0.001). ADAMTS1, A disintegrin and metallopeptidase with thrombospondin motif type 1; 5-Aza-dC, 5-Aza-2′-deoxycytidine.