Figure 2.

Immunocytochemistry identification of glial cells on typical and atypical growth patterns. (A) GFAP⁺ glial cells on the typical growth pattern show different shapes and a random arrangement. (B) Local details of one polarized arranged atypical pattern. The star indicates the atypical pattern; white arrows indicate the polarized orientation of GFAP⁺ cells. (C) Typical pattern top layer O4⁺ cells showing the morphology of oligodendrocyte precursors. (D) Typical pattern top layer GalC⁺ cells (green). Red (GFAP⁺) indicates astrocytes and blue indicates the nuclei of astrocytes. (E) NF⁺ neurites on the typical growth pattern show a freely extending network around DRG neurons. (F) NF⁺ neurites (green) extending along the boundary of the typical pattern and polarized arranged atypical pattern (star); white arrows indicate polarized GFAP⁺ cells (red); arrowheads indicate the neurites avoiding crossing into the atypical pattern. (G) NF⁺ neurites (green) on the typical pattern extend along the boundary of the cobblestone-like atypical pattern. White arrows indicate neurites and the star indicates the GFAP⁺ cobblestone-like atypical pattern with a high cell density. (H) NF⁺ neurites (green) from the typical pattern move away from crossing into the GFAP⁺ (red) cobblestone-like atypical pattern. White arrows indicate neurite direction changes. Scale bar=50 µm. Red, Alexa Fluor^® 594-conjugated secondary antibodies; green, Alexa Fluor^® 488-conjugated secondary antibodies; blue, DAPI staining. GFAP, glial fibrillary acidic protein; DRG, dorsal root ganglia; NF, neurofilament; GalC, galactocerebroside.