Fig. 3.

Effect of Klf4 expression in differentiating c-Kit+ cells. VEGF treatment induced and PDGF decreased Klf4 expression (a, vs. Day 0, dotted line). Overexpression of Klf4 (AdKlf4) increased EC differentiation and reduced SMC markers as shown by tube-formation (b), PCR (c) and western blot (d), as compared to control virus (AdCTL). Klf4 knockdown (ShKlf4) reduced endothelial marker expression and increased levels of SMC markers at RNA (e) and protein level (f) *P < 0.05, **P < 0.001, ***P < 0.0001 vs. control.