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. 2015 May 28;2015:836301. doi: 10.1155/2015/836301

Figure 4.

Figure 4

Quercetin treatment affects complexes OXPHOS properties in mice isolated mitochondria. Heart mitochondria isolated from quercetin-treated mice were digitonin solubilized and fractionated by BN-PAGE and then followed by Complex I (a), Complex II (b), and Complex IV (c) in-gel activity assays. (a) IGA Complex I densitometry analysis for supercomplex and monomer fraction. (b) IGA Complex IV densitometry analysis for supercomplex and monomer fraction. Images show three independent experiments. Each bar represents the mean ± SD, analyzed by two-sample t-test (P < 0.05). Abbreviations used are as follows: blue native polyacrylamide gel electrophoresis (BN-PAGE), Complex I (CI), Complex IV (CIV), in-gel activity assay (IGA), and supercomplexes (SC). SC1:CI:CIII2:CIV1−4; SC2:CIII2:CIV1−4.