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. 2015 Jun 12;14:84. doi: 10.1186/s12934-015-0273-x

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© López et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Color of the different strains constructed. a Color change from strain JLS01, which only expressed the BST1 gene from S. cerevisiae and the carotenogenic genes crtYB and crtI from X. dendrorhous, to JLS02 that additionally expressed the P. hybrida gene PhCCD1. b Color of the different strains expressing episomal versions of the crtYb/crtI/PhCCD1 genes. All the strains expressed the integrated BTS1, crtYB, crtI and Ph CCD1 genes and were subsequently transformed with episomal vectors containing crtYB (JLS3), crtI (JLS04), PhCCD1 (JLS05), crtYB-crtI (JLS06) and crtYB-PhCCD1 (JLS07). All pellets were obtained after 48 h of cultivation in a two-phase culture with 10% dodecane at 20°C. c Representative plate of the constructed strains. Cells were plated after 48 h of cultivation in two-phase culture with 10% dodecane.