Figure 5.

qPCR analysis of the carotenogenic crtYB and crtI genes, and the plant PhCCD1 gene in the different β-ionone producing strains. Three cultures of each strain were inoculated in SC-URA medium with 2% glucose (w/v) and 10% dodecane (v/v), at the same optical density. 48 h later, samples were processed for cDNA synthesis. qPCR experiments were performed to determine the abundance of crtYB, crtI and PhCCD1 in each strain relative to TEF1, which was used as internal control. Results from strains JLS03–JLS07 were compared to JLS02 as a calibrator sample. The data represent the average and the standard deviation of three independently grown cultures.