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The effects of different caspase inhibitors on apoptosis induced by hypericin mediated PDT. SP2/0 Cells were pretreated with caspase pan inhibitor Z-VAD-FMK (100.0 μM) or caspase-3 inhibitor Ac-DEVD-CHO (100.0 μM) for 1 h, and then exposed with 0.05 μM hypericin for 16 hours. At 24 hour after 11.28 J/cm2 irradiation, apoptosis was assayed by flow cytometry after staining with PI. A: control; B: hypericin; C: hypericin + Ac-DEVD-CHO; D: hypericin + Z-VAD-FMK.
