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. 2015 Jun 12;14:83. doi: 10.1186/s12934-015-0272-y

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© Li et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Valinomycin production with different biocatalyst concentrations using EnPresso B defined medium in milliliter-scale cultivations of E. coli BJJ01 pCTUT7-Vlm1 pKS01-Vlm2. a Dissolved oxygen (DO) and b pH were measured online; c cell growth curves; d valinomycin production. An overnight preculture of E. coli BJJ01 pCTUT7-Vlm1 pKS01-Vlm2 (OD₆₀₀ of ~6.0) was distributed without diluting it into the 24-well plates with 1 mL per well (total volume 3.3 mL). Different amounts of biocatalyst (1.5, 3, 9 or 15 U L⁻¹) were added to each well followed by 48 h cultivation at 250 rpm and 30°C with online measurement of DO and pH.