Fig. 3.

ANO1 is the major contributor of CaCC currents in PDAC cells and is sensitive to Cl⁻ channel inhibitors and siRNA. Whole-cell patch-clamp recordings with 1 μM Ca²⁺ in pipette solution. a Representative current traces measured in Capan-1 cells upon exposure to different inhibitors. b Quantification of CaCC currents at +67 mV recorded with inhibitors compared to currents in presence of DMSO; currents of siANO1 cells were compared to those of MOCK transfected cells. c Steady-state I–V relationships of cells transfected with either scrambled siRNA (MOCK) or siRNA targeting ANO1. d Densitometric quantification of Western blot analysis of siRNA knockdown. β-Actin was used as a loading control. (n) = number of individual experiments