Fig. 8.

Cl⁻ channel inhibitors alter ATP-induced Ca²⁺ signals. Intracellular Ca²⁺ measurements in FURA-2 loaded HPDE and BxPC-3 cells. a Representative single-cell traces of individual experiments. Different substances were applied as indicated. ATP was applied at 10 μM, T16A_inh-A01 and CaCC_inh-A01 at 20 μM, and NS3728 at 10 μM. b Summary of maximum ATP-induced changes in [Ca²⁺]_i; for CaCC_inh-A01, these values may be overestimates as the peak response was not present. (n) = number of individual experiments; each containing between 5–45 single cells.***p ≤ 0.001 when compared to no inhibitor condition; ^## p ≤ 0.01 when compared with HPDE no inhibitor