Fig. 9.

ANO1 supports migration of PDAC cells. Wound healing assay is shown as relative wound density (density of wounded area relative to confluency of cell region) over time. Aphidicolin (5 μM) was added to stop proliferation. a AsPC-1 cells and b BxPC-3 cells after transfection with either scrambled siRNA (5 nM) or siRNA targeting ANO1 (50 nM). c BxPC-3 cells in presence of different inhibitors. Data shown represents mean ± s.e.m. of n = 4 experiments; each run was carried out in triplicates