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. 2015 May 28;2015:270328. doi: 10.1155/2015/270328

Figure 3.

Figure 3

Effect of SP on Wnt-related molecules in BMSCs. The mRNA and protein levels of the Wnt pathway proteins β-catenin, GSK-3β/p-GSK-3β, cyclin D1, and c-myc were evaluated by qPCR and Western blot analysis in BMSCs. A significant activating effect of SP on Wnt signaling was observed after 12 h (a). Various concentrations of SP (10−8, 10−10, and 10−12 mol/L) upregulated the protein levels of Wnt pathway proteins (b). An NK-1 antagonist and the Wnt pathway inhibitor DKK1 both attenuated the activating effect of SP on Wnt signaling, indicating that the Wnt pathway mediates the effect of SP (c).