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Effect of Rottlerin on apoptosis and autophagy. Western blotting of PARP and SQSTM1/p62 in Sk-Mel-28 cell lysate after 6–24 h treatment with 20 μM Rottlerin. β-actin was used as loading control. A representative western blot of three independent experiments is shown. Densitometry results are expressed as protein/β-actin ratio.
