Figure 3.

Effect of Rottlerin on NF-κB nuclear translocation. Cells were treated with 20 μM Rottlerin for 6–24 h, stained with anti-p65 NF-κB subunit antibody, and revealed with FITC-conjugated secondary antibody. The merge images of anti-p65 labeling (green) and nuclear staining with DAPI (blue) show that both stainings overlap in the control and after 6 h of Rottlerin treatment, while the p65 expression is restricted in the cytosol after 18–24 h (original magnification 50x). Representative images of two independent experiments.