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Linearity of the multiplex RT-PCR assay using 10-fold dilution series of RNA extracted from an EV-A71 isolate; EV (a) and EV-A71 (b), Cp: crossing point. Concentration used were equivalent to 3 × 101 to 3 × 106 cDNA copies per reaction for EV and 3 × 100 to 3 × 106 cDNA copies per reaction for EV-A71
