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Maximum intra and inter assay variations in different concentrations of plasmid DNA and viral RNA
| Concentration | Plasmid DNA (copies) | Viral RNA (TCID50) | |||||
|---|---|---|---|---|---|---|---|
| 4 × 106 | 4 × 103 | 4 × 101 | 9.6 × 105 | 9.6 × 103 | 9.6 × 101 | ||
| Maximum EV assay co-variation | Intra-assay | 0.53 | 0.93 | 1.37 | 1.49 | 0.84 | 0.77 |
| Inter-assay | 3.13 | 1.36 | 2.18 | 4.41 | 3.01 | 2.54 | |
| Maximum EV-A71 assay co-variation | Intra-assay | 0.52 | 1.00 | 2.18 | 1.34 | 0.73 | 0.73 |
| Inter-assay | 3.19 | 1.37 | 1.85 | 2.66 | 1.56 | 1.53 | |
