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. 2015 May 28;15:55. doi: 10.1186/s12935-015-0204-2

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© Naseri et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Expression level of Bcl2 and Bax proteins in the HepG2, T47D and HCT116 cells. a Following the treatment of the cells with indicated concentrations (IC50 values) and times (24-48 h) of the 3-NC, the cells were harvested and subjected to Western Blot analysis using polyclonal antibodies against Bcl2 and Bax. β-Actin was used as the loading control. b The protein levels of Bcl2 and Bax in control and treated cells were quantified by ImageJ software and normalized to β-actin band intensity. The relative intensities of the proteins over that of Actin for the control cells were set as 100 %. Each data was repeated three times (n = 3)