Fig. 3.

Storage duration of urinary proteins absorbed on the nitrocellulose membrane after SPMA procedure. After urine protein absorption, the membrane was air dried and kept at room temperature until elution at 1-day, 1-week and 1-month. Immediate elution served as control condition. 2-DE was performed to assess protein degradation and/or modification after long storage. No change of urinary proteome was observed up to 1-month