Table 1.
Systematic evaluation on performances of urine preparation methods
| Nitrocellulose-SPMA | Ultrafiltration | Dialysis/lyophilization | 75 % methanol precipitation | |
|---|---|---|---|---|
| Spot number mean ± SD (inter-CV) | 197 ± 15 (7.6 %) | 196 ± 15 (7.6 %) | 192 ± 17 (8.8 %) | 199 ± 17 (8.4 %) |
| %Yield mean ± SD (inter-CV) | 41.9 ± 2.6 (6.4 %) | 42.9 ± 0.6 (1.5 %) | 62.1 ± 8.4 (13.6 %) | 55.6 ± 7.0 (12.6 %) |
| Need of specialized/high-cost instrument | No | No | Yes (lyophilizer) | Yes (superspeed centifuge) |
| Working time (min) | 15 | 35 | >1440 | 65 |
| Step of procedure | 3 | 3 | 3 | 5 |
| Unit cost (US$/5-ml urine) | 0.3 | 6.3 | 0.3 | 1.8 |
Abbreviation: inter-CV inter-assay coefficient of variation