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Strains used in this study, all strains are derived from BY4741 (MATa his3∆0 leu2∆0 met15∆0 ura3∆0), with the additional TRP1 gene knocked out to allow for selection with pCM plasmids
| Strains used in this study | Integrative plasmida |
|---|---|
| BY4741Δtrp1 (MATa his3∆0 leu2∆0 met15∆0 ura3∆0 trp1Δ0) | – |
| BY4741Δtrp1Δrck2 | – |
| BY4741Δtrp1Δrck2 | pCM161 |
| BY4741Δtrp1Δrck2 | pCM161 (RCK2) |
aIntegrative plasmids were constructed as indicated in “Plasmid construction”.
