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. 2015 Jun 10;10(6):e0119860. doi: 10.1371/journal.pone.0119860

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© 2015 Litzlbauer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Average ΔR/R ± SD of a FRET sensor (Twitch-1) expressed in XL1 cells pretreated with poly-L-lysine and ionomycin and subjected to different concentrations of extracellular solution calcium (n = 6 colonies per concentration). (B-C) FRET trace (± standard error of the mean, grey) and individual donor and acceptor traces (± standard error of the mean, grey) of colonies of E. coli XL1 transformed with TN-XXL. Arrows indicate when calcium was applied. (n_100mM = 197 colonies, n_1M = 222 colonies). (D-E) Representative ΔR/R values following calcium application (100 mM) of colonies pretreated with different concentrations of either poly-L-lysine (PL) or ionomycin (IM), while the concentration of the second substance was kept constant. Grey dashed lines represent signal changes after the application of poly-L-lysine or ionomycin, black lines represent additional signal change after the application of calcium, and dark lines indicate total signal changes.