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. 2015 Jun 10;10(6):e0119860. doi: 10.1371/journal.pone.0119860

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© 2015 Litzlbauer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) A commercial spray gun is connected to a laser pointer to allow more reproducible aiming and application of solutions to bacterial colonies. (B) The improved spray protocol reduces variability in ΔR/R of bacterial colonies expressing the FRET calcium sensor Twitch-3. The position of each colony on a plate is indicated by a circle. The recorded ΔR/R value is represented by the diameter of the circle. Note that there is a gradient of ΔR/R values when moving from the center to the periphery.