Table 2. Primer list used in quantitative real-time PCR.
Gene Name | (5') Forward Primers (3') | (5') Reverse Primers (3') |
---|---|---|
Gapdh | GGTGGTCTCCTCTGACTTCAACA | GTGGTCGTTGAGGGCAATG |
Nanog | AGAAGGCCTCAGCACCTAC | GGCCTGATTGTTCCAGGATT |
Oct-3/4 | CTTGAATCCCGAATGGAAAGGG | GTGTATATCCCAGGGTGATCCTC |
Kdr | ACTTTGGAAGACAGAACCAAATTATCTC | TGGGCACCATTCCACCA |
CD31 | GAGTATTACTGCACAGCCTTCA | AACCACTGCAATAAGTCCTTTC |
CD34 | CTACAACACCTAGTACCCTTGGA | GGTGAACACTGTGCTGATTACA |
CD144 | TCACGATAACACGGCCAACA | TGGCATCCCATTGTCTGAGA |
Tie2 | GCTAGAGTCAACACCAAGGCC | TCCAAGAAATCACAGCTGAGGA |
vWF | AGTGCAGACCCAACTTCACC | GTGGGGACACTCTTTTGCAC |
Claudin-5 | CTTCCAGAATGGCAAGAGAGTGA | ACCACTGTTCTCCACTGCTCAGA |
Occludin | ACAAGCGGTTTTATCCAGAGTC | GTCATCCACAGGCGAAGTTAAT |
Zo-1 | TGATCATTCCAGGCACTCG | CTCTTCATCTCTACTCCGGAGACT |
Axin2 | GAGTGGACTTGTGCCGACTTCA | GGTGGCTGGTGCAAAGACATAG |