Table 2. List of primers designed in this study, size of PCR product, target gene, and specificity of primers.
Primer pair | Sequences (5’– 3’) | Size of PCR product (bp) | Target | Specificity a |
---|---|---|---|---|
Mul-15040-F | ATTTTCGCGATTTTGGAGTT | 312 | Hypothetical protein containing a region of putative bacillithiol system oxidoreductase,YpdA family | American mulberry-infecting and Italian olive-associated strains of X. fastidiosa |
Mul-15040-R | TTCTTGTGTACTCCGCCTCA |
aPrimers amplified product only from tested mulberry and olive strains of X. fastidiosa.