Table 3.

m-Values Quantifying Effects of Polyols, Small PEGs, Other Solutes on LacDBD Unfolding and Dissociation of ConA Tetramer

	Lac DBD unfolding m-value (cal·mol−1·molal−1)		concanavalinA tetramer-dimer m-value (cal·mol−1·molal−1)
Solute	Experiment a	Predicted b	Experiment c,d	Predicted b
EG	56.9 ± 11	--
Glycerol	203 ± 18	276 ± 34	170 ± 260	227 ± 37
DiEG	67.0 ± 34	53.8 ± 54		239 ± 53
TriEG	−21.0 ± 29	−14.4 ± 73		411 ± 72
TetraEG	−111 ± 41	−82.6 ± 98 e		583 ± 88 f
PEG300	−253 ± 64	−253 ± 170		1010 ± 180
urea	−440 ± 10 d,g	−512 ± 84	−855 ± 69	−547 ± 120
proline		773 ± 210	598 ± 41	694 ± 310
GB	820 ± 110 d,h	631 ± 470	2160 ± 76	724 ± 520
GuHCl	−747 ± 60 i	−895 ± 170

^aDetermined at 40 °C.

^bPredicted at 25 °C using α-values from Table 2 for glycerol and for PEG end and interior groups, α-values from Diehl et al ³⁷ for urea, proline and GB, and α-values from Pegram et al ³⁹ for GuHCl. In all cases, ASA information is from Table S5; for lacDBD unfolding, an extended-chain model of unfolded lac DBD is used. ³⁶

^cDetermined at 20 °C ²¹

^dExperimental m-values are reported in cal mol⁻¹ M⁻¹. Values in cal mol⁻¹ m⁻¹ will be somewhat smaller in magnitude (except for initial slopes at low solute concentration); differences do not exceed the uncertainties listed.

^ePrediction from 25 °C tetraEG α values in Table 2 is −82.2 ± 79 cal·mol^−1·molal⁻¹

^fPrediction from 25 °C tetraEG α values in Table 2 is 581 ± 91 cal·mol⁻¹·molal⁻¹

^gUrea m-value at 25 °C is −449 ± 11 cal mol⁻¹ M^{−1 59}

^hGB m-value at 25 °C is 780 ± 110 cal mol⁻¹ M^{−1 58}

ⁱExperimental data and α-values are from Pegram et al ³⁹.