A Schematic with the position and morphology of the hermaphrodite
specific neuron (HSN) indicated. Only the left HSN is shown for simplicity. An arrowhead
indicates the characteristic branch formed near the vulva and a box the approximate extent
of the images shown in (B) and (C).
B – C Lateral views with schematics
(B’,C’) of adult animals showing the hermaphrodite-specific serotonergic
neuron HSN. HSN neurons were visualized with zdIs13
(Is[Ptph-1::GFP]). Anterior is to the left, asterisks indicate the
location of the vulva and a scale bar 10 µm.
D – F Quantification of HSNs with branches in
the genotypes indicated. Statistical significance is shown as: **: P<0.005, ***:
P<0.0005, ns: not significant. There is no statistically significant difference in
any pairwise comparison between single and multiple mutants in D. In F, the data for
control and egl-15(n484) is identical to D and E and shown for comparison
only. See Dataset S1 for full
primary data.